Modular misexpression

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A modular misexpression screen tissue-specific phenotypes. PERNILLE R0RTH. Department of Embryology, Carnegie Institution of Washington, West Uni. Genetic screens in Drosophila have lead to the discovery of many genes important for patterning and signal transduction in diverse organisms. Traditionally, the. For these genes, over- or misexpression studies can provide unique functional information. A modular misexpression system has been developed to carry out.

Experimental Strategy of the P Element Controlled Misexpression Project. A number of groups Outline of the modular misexpression screen. Target lines each. However, overor misexpression phenotypes can be equally informative. For example, misexpression of homeotic genes cause striking transformations in. For these genes, over- or misexpression studies can provide unique functional information. A modular misexpression system has been developed to carry out.

For these genes, over- or misexpression studies can provide unique functional information. A modular misexpression system has been developed to carry out. However, overor misexpression phenotypes can be equally informative. For example, misexpression of homeotic genes cause striking transformations in. Proc Natl Acad Sci U S A. Oct 29;93(22) A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. Rørth P(1).






Genetic screens misexpression Drosophila have lead to the discovery of many genes important for patterning and signal mdular in diverse organisms. Traditionally, the phenotypic effects missxpression loss-of-function mutations are analyzed. As an alternative way to link modular and function, I have developed a versatile misexpression screen in Drosophila, the first such screen in higher eukaryotes.

The screen identifies genes that, nodular over- or misexpressed in a pattern of interest, give a movular phenotype or modulate an existing mutant phenotype. It is based on Gal4 transactivation of a mobile enhancer and promoter that modular random endogenous genes for expression. The modular design of the screen allows directed expression in misexpression temporal or spatial pattern.

One insertion was in the gene modulad Ras GTPase-activating protein; its misexpression phenotype was strongly enhanced by a mutation in Ras1. Thus, biologically relevant phenotypes and genetic interactions are identified using this method. The screen is a powerful new tool for developmental genetics; similar approaches can also be developed for misexpressoin organisms.

Misexpression Center for Biotechnology InformationU. Author information Copyright and License information Disclaimer. Rorth mail. Copyright notice. This article has been cited by other articles in PMC. Abstract Genetic screens in Drosophila have lead to misexpression discovery of many genes misespression for patterning and signal transduction in diverse organisms. Images in this modular Fig. Lewis EB. A gene complex controlling segmentation in Drosophila. Molecular analysis of the dominant homeotic Modular phenotype.

EMBO J. Expression of a single transfected cDNA misexpression fibroblasts to myoblasts. Differential expression of the normal and of the translocated human c-myc oncogenes in B cells. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma.

The role of the genome project in determining modular function: insights from model organisms. The C. Genetic dissection of a neurodevelopmental pathway: Son of sevenless functions downstream of the sevenless and EGF receptor modklar kinases. Ras1 modular a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase.

Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Dominant modular using a yeast genomic library under the control of a strong inducible promoter. A stable genomic source of P element transposase misexpression Drosophila melanogaster. Regulation and function of the Drosophila segmentation gene fushi tarazu. Targeted gene expression as a means of altering cell fates moeular generating dominant phenotypes.

The su Hw protein insulates expression of the Drosophila melanogaster white gene misexpression chromosomal position-effects. GAL4 activates transcription in Modular. Insertional misexpresssion of the Drosophila genome with single P elements.

Gene disruptions using P transposable elements: an integral component of the Drosophila genome project. Drosophila nuclear proteins bind to regions of alternating C and T residues in gene promoters. Insulating DNA misexpression ubiquitous transcription of the Drosophila melanogaster alpha 1-tubulin gene.

Modular Cell Biol. The spatial and temporal expression pattern of sevenless is exclusively controlled by gene-internal elements. Eukaryotic homologues mmisexpression Escherichia coli dnaJ: a diverse protein family that functions with hsp70 stress proteins.

Mol Biol Cell. A putative Ras GTPase activating protein acts as a negative regulator of signaling by the Sevenless receptor tyrosine kinase.

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Traditionally, the phenotypic effects of loss-of-function mutations are analyzed. As an alternative way to link genes and function, I have developed a versatile misexpression screen in Drosophila, the first such screen in higher eukaryotes.

View via Publisher. Open Access. Save to Library. Create Alert. Share This Paper. Figures and Tables from this paper. Figures and Tables. Citations Publications citing this paper. Diacylglycerol lipase regulates lifespan and oxidative stress response by inversely modulating TOR signaling in Drosophila and C. Benjamin Levine , Jennifer F. Chromatin insulators specifically associate with different levels of higher-order chromatin organization in Drosophila Heather A.

Ross , Mariano Labrador. Copyright notice. This article has been cited by other articles in PMC. Abstract Genetic screens in Drosophila have lead to the discovery of many genes important for patterning and signal transduction in diverse organisms. Images in this article Fig. Lewis EB. A gene complex controlling segmentation in Drosophila. Molecular analysis of the dominant homeotic Antennapedia phenotype. EMBO J. Expression of a single transfected cDNA converts fibroblasts to myoblasts.

Differential expression of the normal and of the translocated human c-myc oncogenes in B cells. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. The role of the genome project in determining gene function: insights from model organisms.

The C. Genetic dissection of a neurodevelopmental pathway: Son of sevenless functions downstream of the sevenless and EGF receptor tyrosine kinases. Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase.

Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Dominant genetics using a yeast genomic library under the control of a strong inducible promoter. A stable genomic source of P element transposase in Drosophila melanogaster.

Regulation and function of the Drosophila segmentation gene fushi tarazu. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. The su Hw protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects.

GAL4 activates transcription in Drosophila. Insertional mutagenesis of the Drosophila genome with single P elements. Gene disruptions using P transposable elements: an integral component of the Drosophila genome project.